A simple, selective and accurate RP-HPLC method has been developed and validated for the simultaneous estimation of salbutamol sulphate, ambroxol HCl and dextromethorphan HBr in their combined pharmaceutical dosage form. The chromatographic separation was performed on zorbax eclipse plus C18 (100 × 4.6 mm, i.d: 3.5 µm) column. Mobile phase comprised of a mixture of 0.025M phosphate buffer pH 6.4, acetonitrile and triethylamine (70:30:01, v/v/v) at a flow rate 0.8 ml/min. The detection wavelength was set at 224 nm. The calibration curve was linear over the range of 1-5 μg/ml, 5-25 μg/ml, 2-10 μg/ml for salbutamol sulphate, ambroxol HCl and dextromethorphan HBr respectively. The proposed method was validated as per ICH guideline Q2R1 and successfully applied for the estimation of three drugs in combined marketed formulation.
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